Reporter

Part:BBa_K2610043

Designed by: Charlotte de Ceuninck van Capelle, Maaike de Jong   Group: iGEM18_Leiden   (2018-10-10)


pSoxS-mCherry-pKatG-GFP

This composite part features the regulatory part promoter SoxS (BBa_K2610030) coupled to fluorescent protein mCherry (BBa_J06504) as well as the regulatory part promoter KatG (BBa_K2610004) coupled to GFP (BBa_E0040). It can be used to visualize upregulation of SoxS and KatG. The two reporter proteins were selected in such a manner that the fluorophore spectra would not overlap.

The SoxR protein is activated in response to specific types of oxidative stress and acts in the SoxS/SoxR complex. The KatG gene encodes for hydroperoxidase I. It protects aerobic, phosphate-starved cells from oxidative damage. The expression of KatG is activated by the global regulator OxyR.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1075
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1818


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Categories
Parameters
None